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Sensitivity evaluation of the MAIDEN system. (A) Schematic diagram of the two-step operation. (B) Sensitivity of the PbAgo and KmAgo-mediated two-step assay. (C) Schematic diagram of the one-step operation. (D) Sensitivity of the PbAgo and KmAgo-mediated one-step assay. Data were collected from three independent experiments and are presented as the mean ± SD, and significance was considered as ns ≥ 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001.
