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. 2022 Jul 27;13:915095. doi: 10.3389/fmicb.2022.915095

Table 1.

Input values used in our iDynoMiCS biofilm simulations.

Parameter Values Description References
S bulk 10−3−10−2 g/L Bulk concentration of limiting nutrient (here assumed to be oxygen). This value is varied to alter biofilm morphology. Saturation concentration of water at 37oC is 6.6 × 10−3 g/L (Battino et al., 1983)
Y 0.64 g/g Yield—grams of biomass produced per gram of oxygen consumed (Beyenal et al., 2003)
μmax 0.1-0.4 /h Maximum specific growth rate. This value is varied to alter biofilm morphology (Bakke et al., 1984; Robinson et al., 1984; Beyenal et al., 2003; Kragh et al., 2016)
k S 8.12 × 10−4 g/L Concentration of oxygen at which the growth is half maximal (Kragh et al., 2016)
D S 2.3 × 10−4m2/day Diffusion coefficient of nutrient (oxygen) (Stewart, 2003)
Biofilm diffusivity 0.8 Factor multiplying DS to give nutrient diffusion coefficient inside the biofilm (Rittmann and Manem, 1992; Stewart, 2003)
h 80 μm Diffusion boundary layer height (Picioreanu et al., 1998; Xavier et al., 2005; Alpkvist et al., 2006)
ρB 200 g/L Biomass density of microbes in biofilm (Xavier et al., 2005; Bjarnsholt et al., 2009)
r div 2 μm Average radius of microbial agent at division (Beyenal et al., 2003)
k Shov 1.15 Factor multiplying the agent's radius to give the shove radius Default iDynoMiCS value (Lardon et al., 2011)
L y 1,032 μm Width of the simulation domain
N 0 300 Number of initialized microbial agents

These values are loosely based on Pseudomonas aeruginosa in a flow cell type set up (Melaugh et al., 2016).