Alignment of large (α) hydroxylase components in the regions binding the Rieske-type [2Fe–2S] center (a) and in the region purported to bind the mononuclear Fe center (b). MsmA, MSAMO (this work); NdoB, naphthalene dioxygenase (32); NahAc, naphthalene dioxygenase (52); BnzA, benzene 1,2-dioxygenase (26); BedC1, benzene dioxygenase (55); TodC1, toluene dioxygenase (62); BphA1, biphenyl dioxygenase (3); CumA1, cumene dioxygenase (accession no. D37828) (43a); IpbA1, isopropylbenzene dioxygenase (31); BphA, biphenyl dioxygenase (54); BenA, benzoate dioxygenase (40); XylX, toluate dioxygenase (21); CbdC, 2-halobenzoate 1,2-dioxygenase (19); BenA(ec), E. coli homologue of benzoate 1,2-dioxygenase (6); TsaM, toluenesulfonate methyl-monooxygenase (27); VanA, vanillate-O-demethylase (8); Pht3, phthalate dioxygenase (41). The numbering refers to the amino acid sequence of MsmA.