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. 2022 Apr-Jun;23(2):114–119. doi: 10.18502/jri.v23i2.8996

Table 1.

The sequence of oligonucleotides used for detecting genital Mycoplasma

Bacteria Target gene The sequence of oligonucleotides Ampliqon size (bp) Reference
Mycoplasma and Ureaplasma genus 16S rRNA My-in-f : 5’- GTAATACATAGGTCGCAAGCGTTATC-3’ (14, 25)
MGSO-2- r: 5’- CACCATCTGTCACTCTGTTAACCTC-3’ 516
UGSO- r: 5’- CACCACCTGTCATATTGTTAACCTC-3’ 492
M. hominis 16S rRNA RNAH1-f: 5’- CAATGGCTAATGCCGGATACGC-3’ (25)
RNAH2-r: 5’- GGTACCGTCAGTCTGCAAT-3’ 334
M. genitalium 140 kDa adhesion protein MgPa1-f: 5’- AGTTGATGAAACCTTAACCCCTTGG-3’ 282 (25)
MgPa3-r: 5’- CCGTTGAGGGGTTTTCCATTTTTGC-3’
U. urealyticum MB antigen UMS-125-f: 5’ GTATTTGCAATCTTTATATGTTTTCG-3’ (18, 25)
UMA226-r : 5’- CAGCTGATGTAAGTGCAGCATTAAATTC-3’ 448
U. parvum MB antigen UMS-125-f: 5’- GTATTTGCAATCTTTATATGTTTTCG-3’ 403 (18, 25)
UMA226-r: 5’-CAGCTGATGTAAGTGCAGCATTAAATTC-3’