Spotted gar nos3 localization during development. Expression of nos3 is localized in the pharyngeal area in 4 dpf (a,b) and 6 dpf (c,d) embryos, in pharyngeal arches in 7 dpf larvae (e–g) schematized in (h), in developing gills in 11 dpf late larvae (i–l), and in gill lamellae in 14 dpf juveniles (m–p). Coronal (n) and transversal section (o) planes are indicated with a red dashed line in (m). ey, eye; gi, gill; dv, dorsal view; lv, lateral view. Scale bar is 1 mm in (a,c,e,i,m); 100 µm in (b,d,l,n,o,p). (Online version in colour.)