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A) Immunohistochemical staining of Ki67 (scar bar 50 μm) and PCNA (scar bar 100 μm); B) Immunofluorescence staining of CD31 (green) and α-SMA (red), nucleus was stained blue with DAPI, scar bar 40 μm; C) Relative fluorescence of A&B; D) Western blot analysis of tissue from wound area; E) Relative phosphorylation of AKT, ∗p < 0.05 vs. control, ∗∗p < 0.01 vs. control, ∗∗∗p < 0.005 vs. control.
