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. 2022 Jul 21;64:101558. doi: 10.1016/j.molmet.2022.101558

Figure 4.

Figure 4

Co-treatment with senolytic combination Dasatinib plus Quercetin (D + Q) counteracts the senescent expression program induced by insulin. (A) Representative images (bright field) of IHH cells treated with DOX + Insulin or DOX + Insulin + D + Q. Scale bars represent 100 μm. (B) Representative immunoblots of respective proteins in DOX + Insulin-stimulated cells, treated with or without D + Q. Bar graphs showing the relative protein quantification, normalized to GAPDH (n = 7–8). (C) RT-qPCR analysis of senescence markers (p21, CCND1) and SASP factors (IL8, IL18 and IL32) in DOX + Insulin-stimulated cells treated with or without D + Q (n = 6). Data are shown as mean ± SEM. Dots represent individual level data. Statistical significance was determined by Student's t-test or one-way ANOVA followed by Bonferroni post-hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗,###p < 0.001. ∗vs. DOX + Insulin-treated cells; #vs. Ctrl cells. a.u., arbitrary unit.