Supplementary figure 1: Effect on senescence markers in IHH cells following insulin or insulin-like growth factor 1 (IGF1) exposure for 72 h. (a) Representative immunoblots of respective proteins in IHH cells treated with insulin (100 nM) or IGF1 (100 ng/ml) for 72 h. Bar graphs showing the relative protein quantification, normalized to GAPDH (n = 5). (b) RT-qPCR analysis of senescence markers (p16) and SASP factors (IL18, MMP3, IL32 and IL8) in IHH cells treated with insulin (100 nM) or IGF1 (100 ng/ml) for 72 h (n = 5). Data are shown as mean ± SEM. Dots represent individual level data. Statistical significance was determined using one-way ANOVA followed by Dunnett post-hoc test. ∗p < 0.05, ∗∗p < 0.01. ∗vs. Ctrl. a.u., arbitrary unit.