Figure 6.

The SF3B1 ULMs contribute to accurate splicing of endogenous, U2AF2-responsive gene transcripts. The abilities of indicated SF3B1 ULM variants to rescue splicing of representative U2AF2-regulated transcripts, including A, THYN1; B, SAT1; C, INTS13; and D, RNF10, were compared by RT-PCR. The ethidium bromide–stained agarose gels are shown above. The ratios of exon-included:exon-skipped band intensities for background-corrected and averaged replicates were plotted below. Primer sequences are listed in Table S3, and immunoblots are shown in Fig. S4. STD, 100 bp ladder size standards. n.s., not significant; ∗p < 0.05; ∗∗p < 0.005; ∗∗∗p < 0.0005.