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. 2022 Jul 12;298(8):102252. doi: 10.1016/j.jbc.2022.102252

Figure 3.

Figure 3

Immunolocalization analysis of TDP-43 WT-, S375G-, and S375E-expressing clones.A, the following merged immunolocalization plots were reported for each clone where the nuclei (DAPI channel) are stained in blue, whereas the Alexa Fluor 594 red channel detects α-FLAG TDP-43 expression. The count of nuclei with noncanonical shape is reported in a column graph (red for WT samples, blue for S375G, and green for S375E). Statistical analysis was performed with multiple comparison one-way ANOVA test with Bonferroni’s correction using GraphPad software (GraphPad Software, Inc). B, the same analysis was also repeated for each clone following sodium arsenate treatment. DAPI, 4′,6-diamidino-2-phenylindole; TDP-43, TAR DNA-binding protein 43.