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. 2022 Jul 13;1(3):pgac114. doi: 10.1093/pnasnexus/pgac114

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Published by Oxford University Press on behalf of National Academy of Sciences 2022.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Fig. 2. — Direct tranmission of LASV. A single lab-reared M. natalensis was experimentally infected with 1 × 10³ TCID₅₀’s LASV strain Soromba-R via i.m. injection and immediately housed with three naïve cage mates. The rodents were cohoused for 45 days postinfection after which terminal serum samples were collected to assess seroconversion to the LASV nucleocapsid antigen. A total of 10 iterations of the experiment were conducted. Arrows represent the potential for direct contact between the infected rodent and its naïve cage mates, while waves represent hypothetical transmission of virus. * Original animal experimentally infected with LASV Soromba-R. Data shown are reciprocal titers based on ELISA serological analysis using a recombinant LASV nucleocapsid antigen.