Figure 4.

Proliferative CD117^-CRTH2^-CD45RA⁺ ILCs express the transcription factor TCF-1. (A) Intracellular expression of signature transcription factors (Eomes, T-BET, RORγt) in human CD117^-CRTH2^-CD45RA⁺ ILCs, CRTH2⁺ ILC2s, CD117⁺CRTH2^- ILC3s, and NK cells isolated from the spleen of HSPC-engrafted MISTRG mice (n = 5). Matched isotype antibodies were used as controls. (B) Intracellular expression of transcription factors (TCF-1, PLZF) and Ki67 in human CD117^-CRTH2^-CD45RA⁺ ILCs, CRTH2⁺ ILC2s, CD117⁺CRTH2^- ILC3s, and NK cells isolated from the spleen of HSPC-engrafted MISTRG mice (n = 6). Matched isotype antibodies were used as controls. (C) Intracellular IFNγ expression by human ILCs and NK cells in the spleen of HSPC-engrafted MISTRG mice and in umbilical cord blood (n = 4). Cells were either unstimulated or stimulated with PMA and ionomycin for 6 hours. ILCs were gated as human CD45⁺CD127⁺CD94^-CD3^-TCRαβ^-Lin^- cells and NK cells were gated as human CD45⁺CD127^-CD94⁺CD3^-TCRαβ^-Lin^- cells as in Supplementary Figure 1. ILC subsets and NK cells were also gated as CD34^- cells. Data represent mean ± SEM and are representative of two (B, C) or three (A) independent experiments.