Figure 5.

T cell specific deletion of IL-17RA results in increased IL-17A production and reduces expression of IL-17RA on CD4+ and CD8+ T cells but not B cells. A) Flow cytometry was used to measure IL-17RA expression on different white blood cell populations in the spleens of Il17ra^fl/fl mice compared to IL17ra^−/− and Cd4^creIl17ra^fl/fl mice. (n=3 per genotype, representative of 2 experiments). * is statistically (p<0.05) different than WT and ^ is statistically different than Il17ra^fl/fl using a 2way ANOVA followed by Tukey’s Multiple comparisons test. Data are representative of 3 independent experiments. B) Naïve T cells were differentiated into Th17 cells in vitro and the production of IL-17A was measured on day 3 of differentiation by intracellular cytokine staining. (n= 4 per genotype, Error bars represent the SEM.) Statistical significance compared to Il17ra^fl/fl samples was determined with Dunnett’s multiple comparison. Data representative of 3 experiments. *P ≤ 0.05