FIG. 4.

Subcellular localization of MalK. MalK subcellular localization experiments were performed as described elsewhere (20), except that strain ED169 instead of strain ED170 was used and extracts were centrifuged at 20,000 × g instead of 200,000 × g. Under these conditions, all MalK protein was solubilized by Triton X-100, and thus, no additional fractionation of the Triton-insoluble fraction with urea was made. Triton-soluble (s), cytoplasmic (c), and Triton-insoluble (i) fractions were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to immunoblotting. The volume loaded for the Triton-soluble and Triton-insoluble fractions was double that for the cytoplasmic fractions.