Table 1.
Sequences of transcript primers and annealing temperatures for RT-PCR
| mRNA Target | Primer Sequence: 5’-3’ | Annealing temperature | Acession Numbera | Ref |
|---|---|---|---|---|
| GADPH | Forward-GGATGATGTTCTGGAAGAGCC | 55ºC | - | [15] |
| Reverse-AACAGCCTCAAGATCATCAGC | ||||
| IL-1β | Forward-ATGATGGCTTATTACAGTGGCAA | 60ºC | - | [20] |
| Reverse-GTCGGAGATTCGTAGCTGGA | ||||
| TNF-α | Forward-GCTGCACTTTGGAGTGATCG | 60ºC | ||
| Reverse-GTGTGCCAGACACCCTATCT | ||||
| CCL18 | Forward-TGCATTGCAGCGTCATCTTG | 57ºC | NM_002988.4 | - |
| Reverse-GAGTCCCATCTGCTATGCCC | ||||
| CCL22 | Forward-AGGGCCAGGGGACATCTAAT | 57ºC | NM_002990.5 | - |
| Reverse-GAGATCTGTGCCGATCCCAG | ||||
| COX-2 | Forward-GTTCCACCCGCAGTACAGAA | 58ºC | NM_000963.4 | - |
| Reverse-AGGGCTTCAGCATAAAGCGT | ||||
| 5-LOX | Forward-CATGCCCAGGAACAGCTCGTT | 57ºC | - | [21] |
| Reverse-AGTCCTCAGGCTTCCCCAAGT | ||||
| 12-LOX | Forward-GCCCAAAGCTGTGCTAAACC | 58ºC | NM_000697.3 | - |
| Reverse-TGGGGGAGGAAATAGAGCCT | ||||
| 15-LOX | Forward-CCTTCGTCCTCCAAACCTGT | 57ºC | NC_000017.11 | - |
| Reverse-GCTCGGATGTGGGTAGTGAC |
aPrimers have been designed in-house by using Primer Blast from NIH