Fig. 3.

AR-V7 regulate TNC expression and TNC upregulate AR-V7 protein. A VCaP and 22Rv1 cells grown in 5% csFBS containing media were assessed for AR-FL, AR-V7, and TNC transcript expression using RT-qPCR and B protein level using Western blot. C VCaP and 22Rv1 treated with siRNA targeting AR-FL and AR-V7 to evaluate TNC transcript level using RT-qPCR and D protein level using Western blot analysis. E VCaP and 22Rv1 seeded on BSA versus TNC for 72 h in 5% csFBS containing media followed by Western blot analysis of AR-V7 and AR-FL protein expression with β-actin as loading control. F Densitometry quantification of AR-V7 and AR-FL protein expression depicted in (E) for n = 3 biological replicates. N.S. represents no significance. G VCaP and 22Rv1 treated with anti-tenascin monoclonal antibody (BC-24) at increasing concentrations (1, 2.5 μg/ml) and compared to Isotype/IgG control at concentrations (1, 2.5 μg/ml). AR-V7 and AR-FL protein levels were assessed using Western blots. H. Densitometric analysis of Western blot images of AR-V7 expression in VCaP and 22Rv1 treated with IgG or anti-tenascin monoclonal antibody (BC-24) for n = 3 biological replicates. All data represent mean ± SD analyzed by unpaired students t-test (n = 3) *p < 0.05; ** p < 0.01; ***p < 0.001; ****p < 0.0001