Table 2.

Estimating average relative heritable gene expression variation

	CC strain		Diet		CC strain × diet
	Variance	POV (%)	Variance	POV (%)	Variance	POV (%)
All expressed genes	0.069	30.3	0.007	3.9	0.004	2.6
Diet DEGs	0.055	26.2	0.033	18.7	0.004	2.6
Strain DEGs	0.083	36.0	0.007	3.9	0.004	2.6

To estimate the relative heritable variation that can be attributed to genetics, environment (diet), and gene × environmental effects, linear mixed model analyses with CC strain, diet, and CC strain × diet interactions all as random effects were performed to quantify the proportions of variance (POV) attributed to each term relative to each other for the 11,542 expressed genes used in limma differential gene expression analysis. The mean approximate values for proportion of variance for strain, diet, and interaction were calculated by dividing the variance for each term by the sum of the variance for all terms in the model and multiplied by 100