Fig. 1.

Sf9-baculovirus-purified kinesin-3 motors are robust and superprocessive. A–E Fluorescently tagged truncated constitutively active motors were purified using Sf9-baculovirus expression system. In vitro single-molecule motility assays of Sf9-purified A kinesin-1, KHC (1–560) and B–E kinesin-3 motor B KIF1A(1-393LZ), C KIF13A(1-411ΔP), D KIF13B(1-412ΔP), and E KIF16B(1–400) motors. For each population of motors, histograms of velocities (left panel) and run lengths (right panel) were plotted and fit to a single Gaussian. Average velocity and run length of the corresponding population of motors (N) are indicated on top-right or left corner as mean ± SEM. Data presented from three independent experiments