Fig. 4.

Upregulation of NLRP3 and activated caspase-1 in the spinal cord of ALS mice with disease progression. mRNA expression of NLRP3 at 60 d, 95 d, 108 d and 122 d of ALS mice, compared with CON mice a. Protein expression of NLRP3 at 60 d, 95 d, 108 d and 122 d in ALS mice b. mRNA expression of caspase-1 at 60 d, 95 d, 108 d and 122 d of ALS mice, compared with CON mice c. Caspase-1 activation from the pre-symptomatic stage to the end stage in ALS mice d. Data represent means ± SEM (n = 4). ^*P < 0.05, ^**P < 0.01