Fig. 8.

Cell specific location of IL-1β in the lumbar spinal cord. Representative images of double immunofluorescence staining for IL-1β with NeuN, GFAP, or Iba1 are shown. At 60 d, increased expression of IL-1β was observed in the neuronal cytoplasm of ventral horn spinal cord of ALS mice, and low expression was found in CON mice a. Besides NeuN⁺/IL-1β⁺ double positive cells, dramatic co-localization of IL-1β with activated GFAP⁺ astrocytes and Iba1⁺ microglia in transgenic ALS mice were observed from 95 to 122 d b, c, d. Arrows indicate double-labelled cells. Scale bar 50 μm