Figure 5:

Considerations for collection of tissues for long-read sequencing of nonmodel organisms. General representation of a sequencing pipeline and considerations that may directly or indirectly affect the quality of sequencing output. Stars indicate particular sources of variation manipulated in this study. Several logistical aspects need to be considered prior to sample collection for uHMW DNA isolation with the goal of producing reference-quality genomes. The collector needs to identify what tissue types can be collected from the target species, what preservation methods and cold storage are available, and how quickly samples can be transported to a –80°C ultra-cold freezer.