Figure 1.

Modular covalent decoration of the adenovirus capsid via insertion of DogTag into hexon HVR loops

(A) Modular display of DogCatcher-fused antigenic ligands on the surface of the adenovirus capsid via covalent coupling with DogTag inserted into hexon HVR surface loops. Attachment of antigens to the capsid achieved by simple co-incubation of adenovirus and antigen components in a rapid and spontaneous reaction. (B) Design of modified adenovirus hexon sequences with DogTag inserted into either HVR1, HVR2, or HVR5 flanked by flexible linkers. Amino acid residue numbers corresponding to deletion/insertion sites at HVR1, HVR2, or HVR5 in Ad5 hexon are indicated.