Figure 3.

THP-1 BLUE NFκB responses to TLR ligands and various antibodies with different amounts of aggregation. (A) SEAP reporter gene production in response to TLR ligands are similar with regards to cytokine/chemokine production responses. THP-1 BLUE NFκB cells were treated with varying concentrations of the TLR4 ligand LPS or the TLR2/TLR6 ligand FSL-1. 24 hrs later, supernatant was removed for quantitation of SEAP activity and cytokines/chemokines. The results are normalized to the signal from the vehicle control. Results are the average of 3 independent experiments. (B) THP-1 BLUE NFκB responses to stressed antibodies. THP-1 BLUE NFκB cells were treated with stir stressed and unstressed trastuzumab, infliximab, or mAb1 drug product that had been stored at -80°C or at 25°C for 12 months. The cells were treated with three different concentrations. Results are the average of 3 independent experiments. Error bars represent S.E.M.