Table 1.

Microflow imaging analysis of antibody aggregates: Comparison of spontaneously forming aggregates from upstream processing.

Sample	2-5 µm	5-10 µm	10-25 µm	25-50 µm
mAb1 FNVI	11,121	3,729	1,380	60
mAb1 AEX	1,526	472	224	25
mAb1 VFP	1,824	427	158	16
Trastuzumab	1,010	330	178	6
Trastuzumab Stirred	502,730	83,950	11,886	36

Sub-visible particles were characterized using an MFI 5100 (Protein Simple) instrument equipped with a 400 µm flow cell. The particle size range that can be characterized is from 2 to 300 microns. A 5 µm size standard was run prior to testing. Samples were diluted from 0.01 to 1 mg/mL in water. A sample volume of 900 µL was analyzed per sample. Per 1 mg.