Table 2.
Enzymatic properties of CE orthologs from different species.
| CE | Optimum pH | Optimum temperature (°C) | Specific activity (epimerization) (U mg−1) | Specific activity (isomerization) (U mg−1) | Ratio (E/I) | Tm | Lactose |
Cellobiose |
Reference | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| kcat (s−1) | Km (mM) | kcat/Km (mM−1 s−1) | kcat (s−1) | Km (mM) | kcat/Km (mM−1 s−1) | ||||||||
| Dith-CE | 7 | 75 | 160.1 ± 6.5 | 3.52 ± 0.23 | 45 | 92.15 | 441.4 | 5232 | 0.844 | 320.0 | 354.9 | 0.902 | This study |
| Thth-CE | 7 | 60 | 82.1 ± 3.1 | Less than 0.08 | 70.3 | 200 | 420.5 | 0.478 | 247.0 | 186.0 | 1.328 | This study | |
| Casa-CEa | 7 | 65 | 90.4 ± 4.8 | 2.38 ±0.18 | 38 | 84.14 | 229.8 | 491.1 | 0.468 | 227.2 | 350.7 | 0.648 | This study |
| Caob-CE | 7.5 | 70 | 118 | 2.81 | 42 | 86.7 | 233.4 | 301.7 | 0.773 | 233.7 | 164.5 | 1.421 | 25 |
| Rual-CE | 7.5 | 30 | 38.8b | 0.001b | 38,800 | - | 52.1 | 33 | 1.6 | 63.8 | 13.8 | 4.6 | 26 |
| Bafr-CE | 7.5 | 45 | 76.8b | 0.0005b | 153,600 | - | 79.5 | 6.56 | 12.1 | 67.6 | 3.75 | 18 | 27 |
| Fljo-CE | 8.4 | 35 | 48.5b | 0.0005b | 97,000 | - | 17.5 | 34.9 | 0.501 | 39.9 | 53.2 | 0.75 | 28 |