Table 1.
Primers used in this study, with originating loci, sequences and references.
| Locus | Product name | Primer name | Direction | Sequence (5’–3’) | Reference |
|---|---|---|---|---|---|
| act | Actin | ACT-512F | Forward | ATGTGCAAGGCCGGTTTCGC | Carbone & Kohn (1999) |
| ACT-783R | Reverse | TACGAGTCCTTCTGGCCCAT | Carbone & Kohn (1999) | ||
| apn2 | Mat1 and the adjacent DNA lyase gene | Apn1W1F | Forward | ATGGAGCACAAAAACGAACA | Crouch et al. (2009b) |
| Apn1W1R | Reverse | GCGGAGCAGAGGATGTAGTC | Crouch et al. (2009b) | ||
| cal | Calmodulin | CL1C | Forward | GAATTCAAGGAGGCCTTCTC | Weir et al. (2012) |
| CL2C | Reverse | CTTCTGCATCATGAGCTGGAC | Weir et al. (2012) | ||
| chs-1 | Chitin synthase | CHS-79F | Forward | TGGGGCAAGGATGCTTGGAAGAAG | Carbone & Kohn (1999) |
| CHS-345R | Reverse | TGGAAGAACCATCTGTGAGAGTTG | Carbone & Kohn (1999) | ||
| ApMat | Apn2-Mat1-2 intergenic spacer and partial mating type Mat1-2 gene | AMF1 | Forward | CCAGAAATACACCGAACTTGC | Silva et al. (2012) |
| AMR1 | Reverse | TCATTCTACGTATGTGCCCG | Silva et al. (2012) | ||
| gapdh | Glyceraldehyde-3-phosphate dehydrogenase | GDF1 | Forward | GCCGTCAACGACCCCTTCATTGA | Guerber et al. (2003) |
| GDR1 | Reverse | GGGTGGAGTCGTACTTGAGCATGT | Guerber et al. (2003) | ||
| gs | Glutamine synthetase | GSF1 | Forward | ATGGCCGAGTACATCTGG | Guerber et al. (2003) |
| GSR1 | Reverse | GAACCGTCGAAGTTCCAC | Guerber et al. (2003) | ||
| GSLF2 | Forward | TACACGAGSAAAAGGATACGC | Liu et al. (2016) | ||
| GSLR1 | Reverse | AGRCGCACATTGTCAGTATCG | Liu et al. (2016) | ||
| his3 | Histone3 | CYLH3F | Forward | AGGTCCACTGGTGGCAAG | Crous et al. (2004) |
| CYLH3R | Reverse | AGCTGGATGTCCTTGGACTG | Crous et al. (2004) | ||
| ITS | Internal transcribed spacer | ITS1 | Forward | TCCGTAGGTGAACCTGCGG | White et al. (1990) |
| ITS4 | Reverse | TCCTCCGCTTATTGATATGC | White et al. (1990) | ||
| Mat1/Apn2 | The 3′ end of apn2 and the 5′ end of the mating type gene Mat1-2 | Mat1M72F | Forward | ACGGCAAACGGCTCAGGGAGTG | Crouch et al. (2009b) |
| Mat1M72R | Reverse | AATGCCGAGTCCCACGAGGTTCG | Crouch et al. (2009b) | ||
| sod2 | Manganese-superoxide dismutase | SOD625F | Forward | GCCCACAGTACATATTGCCTAAGC | Crouch et al. (2006) |
| SOD625R | Reverse | TCATCCCGGGAGCCAGAAAACCT | Crouch et al. (2006) | ||
| tub2 | β-tubulin 2 | T1 | Forward | AACATGCGTGAGATTGTAAGT | O’Donnell & Cigelnik (1997) |
| Bt2b | Reverse | ACCCTCAGTGTAGTGACCCTTGGC | Glass & Donaldson (1995) |