Table 1.

Differences in the preparation of each data set

	Condition 1	Condition 2	Condition 3	Condition 4
Operator	Operator 1	Operator 1	Operator 2	Operator 3
Cell growth and preparation protocol	Sun et al.	Sun et al. with modified growth	Sun et al.	Sun et al.
Lysis method	French Press	Microfluidizer (Microfluidics M110-P)	Microfluidizer (Microfluidics M110-P)	Microfluidizer (Microfluidics M110-P)
Lysate prep location	UMN	CBC	CBC	CBC
Lysate batch	UMN	CBC-1	CBC-2	CBC-2
Supplement mix prep location	UMN	CBC	CBC	CBC
Supplement mix prep batch	UMN	CBC-1	CBC-2	CBC-2
DNA prep kit	Promega Plus Wizard Midi Kit	Promega Plus Wizard Midi Kit	Qiagen Plasmid Midi Kit	Qiagen Plasmid Midi Kit
DNA prep location	CBC	CBC	CBC	CBC
DNA prep batch	CBC-1	CBC-1	CBC-2	CBC-2
GFP standard prep batch	sfGFP-1	sfGFP-1	eGFP-1	eGFP-1
Dispensing method	Acoustic Liquid Handling (Labcyte Echo 525)	Acoustic Liquid Handling (Labcyte Echo 525)	Manual	Manual
Plate	96-Well Clear V-bottom Polypropylene plates (Costar Cat# 3357)	96-Well Clear V-bottom Polypropylene plates (Costar Cat# 3357)	96-Well round bottom plates (Immulon 2HB U Bottom Thermo Fisher Cat# 3655)	96-Well round bottom plates (Immulon 2HB U Bottom Thermo Fisher Cat# 3655)
Plate seal	Storage Mat for 96-well Polypropylene plates, Costar Cat# 3080	Storage Mat for 96-well Polypropylene plates, Costar Cat# 3080	3 M Empore Sealing Tape MG Sci. Cat # E520-43	3 M Empore Sealing Tape MG Sci. Cat # E520-43
Reaction prep location	CBC	CBC	CBC	CBC
Plate readera	BioTek Synergy H1A/BioTek Synergy HTb	BioTek Synergy H1A	BioTek Synergy H1B/BioTek NEO	BioTek Synergy H1B/BioTek NEO

^aTwo instruments of the same type (BioTek Synergy H1) were used and are indicated by A or B. See Table S2 for which circuit was tested on each plate reader.

^bNo GFP standard curve at 5 µl is available for the data on the BioTek Synergy HT.