TABLE 2.

Primers used for PCR amplification of chromosomal DNA

Primer	Location	Sequencea
JD109	wza	5′-AGCGACTACGATTTGGATAAGC
JD89	wzc	5′-ACGGGCAATAACAGGAAAATA
JD90	wcaO	5′-TATGTGGGGCGATTGACTCC
JD53	wzx	5′-TTTCCGAAACACGCTTAAAA
JD95	orfX	5′-TGCGCTTCCACATACCAGTTA
JD99	orfX	5′-GAAAGCAAGCCCAATGTCAC
GALF1	galF	5′-GGGCGATCTCTCCGAATACTC

^aPrimer sequences were based on the relevant chromosomal region from E. coli E69 (17) (GenBank accession no. AF104912) and, for GALF1, K. pneumoniae Chedid (2) (GenBank accession no. D21242).