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. Author manuscript; available in PMC: 2023 Aug 2.
Published in final edited form as: Cell Metab. 2022 Aug 2;34(8):1201–1213.e5. doi: 10.1016/j.cmet.2022.07.006

Figure 7. Iron enrichment induces NASH-associated ROS accumulation in HSCs.

Figure 7.

(A) Effect of FAC treatment on ROS content in healthy human HSCs.

(B) ROS abundance in HSCs isolated from 24wks WD fed WT and Rab27ΔHep mice.

(C) Effect of Hep EV treatment on HSC ROS levels in lean KC-KO mice.

(D) Effect of ROS chelator treatment on the expression of fibrogenic genes in FAC-treated healthy human HSCs.

HSC ROS content (E), fibrogenic gene abundance (F), and liver Sirius Red staining (G) after switching NASH WT mice to NCD diet for 9wks. Representative images of n=4–6 mice per group.

Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test (A, B, D–G) or One-way ANOVA (C). See also Figure S7.