KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-HIF2α | ABclonal | Cat# A7553; RRID: AB_2768078 |
| Anti-ATF4 | ABclonal | Cat# A18687; RRID: AB_2862422 |
| Anti-Rab27a | Cell signaling technology | Cat# 69295; RRID:AB_2799759 |
| Anti-Rab27b | ABclonal | Cat# A8897; RRID: AB_2771907 |
| Anti-HSP90 | Santa Cruz | Cat# sc-101494; RRID: AB_1124018 |
| Anti-Phospho-AKT Ser473 | Cell signaling technology | Cat# 9271S; RRID: AB_329825 |
| Anti-pan AKT | Cell signaling technology | Cat# 4691S; RRID: AB_915783 |
| Anti-FPN | ABclonal | Cat# A14884; RRID: AB_2761764 |
| Anti-FtH | ABclonal | Cat# A1144; RRID: AB_2758562 |
| Anti-Cd11b-FITC | BioLegend | Cat# 101205; RRID: AB_312788 |
| Anti-F4/80-PE Cy7 | BioLegend | Cat# 123113; RRID: AB_893490 |
| Anti-Cd45-APC Cy7 | BioLegend | Cat# 103115; RRID: AB_312980 |
| Anti-Desmin, eFluor 660 | eBioscience | Cat# 50-9747-80; RRID: AB_2574355 |
| Anti-phospho-eIF2a | Cell signaling technology | Cat# 3398; RRID: AB_2096481 |
| Anti-α-SMA (for immunofluorescent staining assays) | eBioscience | Cat# 14-9760-82; RRID: AB_2572996 |
| Anti-α-SMA (for Western blots) | Sigma Aldrich | Cat# A5228-25UL; RRID: AB_262054 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Live/Dead Fixable Aqua dead cell stain kit | ThermoFisher | Cat# L34966 |
| Novolin R regular human insulin used in ITTs | Novo-Nordisk | Cat# NDC 0169-1833-11 |
| Sodium acetate (1-14C) | APC0101A-50 μCi | American Radiolabeled Chemicals, Inc |
| Dextrose | Hospira, Inc | Cat# 0409-6648-02 |
| DT (Diphtheria Toxin) | Bio Academia | Cat# 01-517 |
| Percoll | GE Healthcare Life Sciences | Cat# 17-0891-01 |
| SuperSignal™ West Femto Maximum Sensitivity Substrate | ThermoFisher Scientific | Cat# 34095 |
| Halt Protease and Phosphatase Inhibitor Cocktail | ThermoFisher Scientific | Cat# 78440 |
| RIPA buffer (10x) | Cell Signaling Technology | Cat# 9806 |
| AIN76A Western Diet | TestDiet | Cat# 5342 |
| Collagenase D | Roche | Cat# 11088882001 |
| Pronase | Roche | Cat# 11459643001 |
| DNAnase I | Roche | Cat# 10104159001 |
| RBC lysis buffer | eBioscience | Cat# 00-4333-57 |
| High-capacity cDNA reverse transcription kit | ThermoFisher Scientific | Cat# 4368813 |
| iTaq SYBR Green supermix | Bio-Rad | Cat# 172-5125 |
| Exosome-depleted FBS | SBI | Cat# EXO-FBSHI-50A-1 |
| Lipofectamine RNAiMAX reagent | ThermoFisher Scientific | Cat# 13778-075 |
| Empty liposome | FormuMax | Cat# F70101-NH |
| Iron-Dextran | Sigma-Aldrich | Cat# D8517 |
| FAC (Ammonium iron citrate) | Sigma-Aldrich | Cat# F5879 |
| DFO (Deferoxamine mesylate salt) | Sigma-Aldrich | Cat# D9533 |
| Holo-transferrin | Sigma-Aldrich | Cat# T0665 |
| Apo-transferrin | Sigma-Aldrich | Cat# T5391 |
| Palmitic acid | Sigma-Aldrich | Cat# P5585 |
| Critical Commercial Assays | ||
| Quick-RNA microprep kit | Zymo research | Cat#R1051 |
| Triglyceride colorimetric assay kit | Cayman | Cat# 10010303 |
| Iron Assay Kit | Sigma Aldrich | MAK025 |
| Mouse Hepcidin ELISA Kit | BioVision | E4693-100 |
| ROS-ID Total ROS/Superoxide Detection Kit | Enzo Life Sciences | ENZ-51010 |
| Deposited Data | ||
| RNA seq data | GEO database | GSE185694 |
| Experimental Models: Organisms/Strains | ||
| Mouse: WT C57BL6/J | Jackson Laboratories | JAX: 000664 |
| Mouse: ATF4 flox/flox | Michael Karin lab | JAX: 006366 |
| Mouse: Rosa26-floxed STOP-Cas9 | Jackson Laboratories | JAX: 026175 |
| Mouse: Albumin cre | Jackson Laboratories | JAX: 003574 |
| Mouse: B6-iDTR, ROSA26iDTR | Jackson Laboratories | JAX: 007900 |
| Mouse: C57BL/6-Lratem1(2A-Cre)Smoc | Shanghai Model Organisms | NM-KI-190097 |
| Mouse: Clec4f-Cre-tdTomato | Jackson Laboratories | JAX: 033296 |
| Oligonucleotides | ||
| siRNA control | Horizon Discovery | Cat# D-001810-01-05 |
| siRNA-human Hif2a | Horizon Discovery | Cat# J-004814-06-0002 |
| siRNA-human ATF4 | Horizon Discovery | Cat# J-005125-10-0002 |
| siRNA-mouse Hif2a | Horizon Discovery | Cat# J-040635-09-0002 |
| siRNA-mouse Rab27a | Horizon Discovery | Cat# J-060970-09-0002 |
| siRNA-mouse Rab27b | Horizon Discovery | Cat# J-050808-09-0002 |
| siRNA-human Rab27a | Horizon Discovery | Cat# J-004667-05-0002 |
| siRNA-human Rab27b | Horizon Discovery | Cat# J-004228-05-0002 |
| Cy3-labeled miR-223 mimic | GE Dharmacon | Cat# SO-2465930G |
| gRNA-Rab27a plasmid | Sigma Aldrich | Sanger Clone ID: MM5000028879 |
| gRNA-Rab27b plasmid | Sigma Aldrich | Sanger Clone ID: MM5000025441 |
| gRNA-Tfrc1 plasmid | Sigma Aldrich | Sanger Clone ID: MMPD0000041027 |
| gRNA-Fpn plasmid | Sigma Aldrich | Sanger Clone ID: MMPD0000046431 |
| Software and Algorithms | ||
| Prism | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| FlowJo | FlowJo | https://www.flowjo.com/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Image Lab | Bio-Rad | https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z |