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. Author manuscript; available in PMC: 2023 Jul 15.
Published in final edited form as: J Immunol. 2022 Jun 27;209(2):227–237. doi: 10.4049/jimmunol.2100558

Figure 2. Increased negative selection of developing autoreactive CD8 T-cells with both ablation and overexpression of Nfkbid.

Figure 2.

(A) Flow cytometric gating strategy to quantify CD4+CD8+ DP thymocytes expressing the AI4 TCR using antibodies for TCRVα8.3 and TCRVβ2 in conjunction with AI4 tetramer binding. (B) Quantification of AI4 DP thymocytes from NOD-AI4, NOD-AI4-Nfkbid−/−, and NOD-AI4-60A mice (n= 9–13 mice per group). Statistical significance was determined by Mann-Whitney nonparametric comparison. (C) RNAseq was performed on DP thymocytes from NOD-AI4, NOD-AI4-Nfkbid−/− and NOD-AI4-60A mice. Among the differentially expressed genes, those harboring NF-κB transcription factor binding sites within promoter regions were identified.