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. Author manuscript; available in PMC: 2023 Jul 15.
Published in final edited form as: J Immunol. 2022 Jun 27;209(2):227–237. doi: 10.4049/jimmunol.2100558

Figure 3. Nfkbid overexpression increases the peripheral Treg compartment.

Figure 3.

(A) Nfkbid mRNA expression was measured by qPCR and normalized to Gapdh expression in CD4+CD25+ Tregs sorted from the spleens of 6–8-week-old female NOD, NOD-Nfkbid−/− and NOD-60A mice (n=3–5 per strain). (B, C) Frequency of Foxp3+ Tregs among CD4 T-cells as measured by flow cytometry in spleens (B) and Panc LN (C) from 6–8-week-old female NOD, NOD-Nfkbid−/− and NOD-60A mice (n=8 per strain). (D, E) Frequency of mature CD25+Foxp3+ Tregs among CD4+ single positive thymocytes from NOD, NOD-Nfkbid−/− and NOD-60A mice and frequency of CD25+Foxp3− and CD25−Foxp3+ developing Tregs as measured by flow cytometry in 6–8-week-old female mice (n=8 per strain). Statistical significance in all panels was determined by Mann-Whitney nonparametric comparison.