Alignment of the deduced amino acid sequences of copper-containing nitrite reductases from different bacterial sources. The amino acid alignment was generated with the CLUSTAL W package (34). Asterisks represent amino acid identity, and dots indicate similar amino acids. Similar amino acids include aromatic amino acids, basic amino acids, acidic amino acids, and hydrophobic amino acids. The numerals 1 and 2 above the sequence alignment signify the type 1 and type 2 copper ligands, respectively. ALCXY, A. xylosoxidans accession number AF051831; ACHCY, A. cycloclastes accession number Z48635 (9); ALCFA, A. faecalis accession number D13155 (22); PSEsp, Pseudomonas sp. strain G-179 accession number M97294 (41); PSEAR, P. aureofaciens accession number Z21945 (10); RHIHE, R. hedysari accession number U65658 (35); RHOSPH, R. sphaeroides accession number U62291 (36).