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. 2022 Jan 24;28(7):1446–1459. doi: 10.1158/1078-0432.CCR-21-1846

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©2022 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.

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Figure 4. DNA-PK regulates glycolysis. A, Pyruvate levels and lactate production was measured upon DNA-PK inhibition (NU7441, 1 μmol/L, 24 hours). B, DNA-PK knockdown (96 hours posttransfection). C, Pyruvate levels upon overexpression of ALDOA and PKM2 as validated via protein levels (left). D, Glucose tracing through glycolysis pathway to GADP, 3PG, pyruvate, and lactate generation through metabolic flux analysis. C4–2 cells were treated with NU7441 (1 μmol/L, 24 hours) or vehicle control, followed by 2 hours pulse with [U-13C6]-D-glucose. Data collected in at least three biological replicates, represented as mean ± SEM (ns, nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001). — DNA-PK regulates glycolysis. A, Pyruvate levels and lactate production was measured upon DNA-PK inhibition (NU7441, 1 μmol/L, 24 hours). B, DNA-PK knockdown (96 hours posttransfection). C, Pyruvate levels upon overexpression of ALDOA and PKM2 as validated via protein levels (left). D, Glucose tracing through glycolysis pathway to GADP, 3PG, pyruvate, and lactate generation through metabolic flux analysis. C4–2 cells were treated with NU7441 (1 μmol/L, 24 hours) or vehicle control, followed by 2 hours pulse with [U-¹³C₆]-D-glucose. Data collected in at least three biological replicates, represented as mean ± SEM (ns, nonsignificant; *, P < 0.05; **, P < 0.01; ***, P < 0.001).