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. 2022 Mar 14;28(6):1147–1156. doi: 10.1158/1078-0432.CCR-21-2023

Figure 4.

Figure 4. Associations of FcγRI+ macrophages in cHL tumor microenvironment with clinical outcomes of tislelizumab. A, Representative figure showing multiplex immunofluorescence staining (20× resolution) for CD8 T cells (CD8+ cells), total Fcγ receptor I (FcγRI+ cells), macrophages (CD68+ cells), FcγRI+ macrophages (FcγRI; CD68 double-positive cells), PDL1+, and CD30+ cells. B, CD8+ T cells, FcγRΙ+ macrophage infiltration, and their associations with complete response rates to tislelizumab. C, PFS by CD8+ T cells and FcγRΙ+ macrophages, divided by median cell percentage value of CD8 T cells and FcγRΙ+ macrophages separately. All mIHC images were acquired using Leica Aperio VERSA 8 auto-scanner. Note: (i) Make and model of microscope: LEICA DM6 B; (ii) Type, magnification, and numerical aperture of the objective lenses: ∞/0.17/OFM25/C, HC PL APO, 20×/0.80; (iii) Temperature: 20°C–30°C; (iv) Imaging medium: sCMOS: 23CAM014; (v) Fluorochromes: PerkinElmer Opal automation Multiplex IHC kit; (vi) Camera make and model: Andor Zyla 5.5; (vii) Acquisition software: Aperio VERSA. CR, complete response.

Associations of FcγRI+ macrophages in cHL tumor microenvironment with clinical outcomes of tislelizumab. A, Representative figure showing multiplex immunofluorescence staining (20× resolution) for CD8 T cells (CD8+ cells), total Fcγ receptor I (FcγRI+ cells), macrophages (CD68+ cells), FcγRI+ macrophages (FcγRI; CD68 double-positive cells), PDL1+, and CD30+ cells. B, CD8+ T cells, FcγRΙ+ macrophage infiltration, and their associations with complete response rates to tislelizumab. C, PFS by CD8+ T cells and FcγRΙ+ macrophages, divided by median cell percentage value of CD8 T cells and FcγRΙ+ macrophages separately. All mIHC images were acquired using Leica Aperio VERSA 8 auto-scanner. Note: (i) Make and model of microscope: LEICA DM6 B; (ii) Type, magnification, and numerical aperture of the objective lenses: ∞/0.17/OFM25/C, HC PL APO, 20×/0.80; (iii) Temperature: 20°C–30°C; (iv) Imaging medium: sCMOS: 23CAM014; (v) Fluorochromes: PerkinElmer Opal automation Multiplex IHC kit; (vi) Camera make and model: Andor Zyla 5.5; (vii) Acquisition software: Aperio VERSA. CR, complete response.