Author response image 1. Changes in instantaneous diffusion coefficient as a function of position along the DNA substrate.

(A) The AT-content along the Λ phage genome is plotted in both orientations, revealing an asymmetric distribution of AT-content directionally across the genome. Averaging the AT-content across the two orientations shows that the center of the DNA has a slightly higher AT-content, indicating that if there is any sequence correlation to SWR1 diffusion, we may see a difference in mean diffusion coefficients at the center of the DNA (since the DNAs in this study were not intentionally oriented). (B) The instantaneous diffusion coefficient of SWR1 as a function of position along the DNA substrate. The center of the DNA substrate does not appear to exhibit any enrichment for higher or lower mobility in the SWR1 remodeler. Nonetheless, the experimental set-up would need to be further optimized to determine if there is in fact no correlation with sequence or if there is simply not enough resolution in our current set-up to discern one.