Fig. 1. Expression of substance P in normal and alopecia areata (AA) scalp (n=9; scale bar: A, 200 µm; B, 100 µm). (A) Paraffin-embedded normal scalp skin and AA lesions are immunohistochemically stained with anti-substance P. Examination of the expression of substance P in the interfollicular epidermis. (B) Paraffin-embedded normal scalp skin and AA lesions are immunohistochemically stained with anti-substance P. Examination of the expression of substance P in the hair follicles. (C) Quantification of the relative staining area. ^*Statistically significant (p<0.05).