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. 2022 Aug 8;219(9):e20212311. doi: 10.1084/jem.20212311

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© 2022 Karo-Atar et al.

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Figure S2. — Fetal-like reprogramming is partially Yap dependent and is not amplified by IFNγ. Related to Figs. 1, 2, and 3. (A) Immunohistochemistry for active YAP; squares, area in bottom panel; open arrowheads, nuclear YAP expression; black arrowheads, worm presence. (B–D) qPCR analysis of the indicated genes from Yap^−/− and littermate control SI organoids stimulated with Hpb-CM for 24 h. (E–G) qPCR analysis of Clu^−/− and littermate control SI organoids stimulated with Hpb-CM for 24 h. (H–K) Vil^cre;Yap^fl/fl and littermate control Vil^cre;Yap^fl/+ mice were infected with 150 L3 Hpb larvae, and adult worms were counted 28 dpi. (I–K) qPCR analysis of the indicated genes from SI organoids stimulated, on day 0 of culture, with ENR (control) or 10 ng/ml IFNγ with or without Hpb-CM for 24 h. (M) Monocle 3 direct exports of the trajectory analysis using CBCs as the pseudotime source variable, showing the distribution of the different cell clusters along the pseudotime variable. Scale bar, 100 μm. Data shown are representative of two or more independent experiments, n > 3 biological replicates; statistical tests: two-way ANOVA (B–L); *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.001.