Figure S3.

A balanced regulation of the ISC niche by helminths and type 2 immune signaling supports durable infection. Related to Fig. 5. (A) Representative photomicrographs of Clu RNAscope of Il4ra^WT and Il4ra^ΔIEC tissue sections on day 14 after Hpb infection. (B) Representative photomicrographs of dtTomato expression in Clu fate-mapping mice treated with PBS or IL4C as described in Fig. 5 Q and Materials and methods. (C) Hpb worm burden 28 dpi in WT and Stat6^−/− mice. (D) Hpb egg burden following IL4C treatment. (E–J) Immune phenotyping on day 14 after Hpb infection. SI (E), PPs (F), and MLNs (G) were harvested from WT and Stat6^−/− mice. Single-cell suspensions were made, and flow cytometry was performed. Cells were gated for viable CD45⁺CD3⁺B220⁻CD4⁺CD62L⁻CD44^high cells, and Gata3⁺ cells were identified as Th2 cells. Percentage of Gata3⁺ cells of the CD62L⁻CD44⁺ population. (H–J) SI (H), PPs (I), and MLN (J) were harvested from Il4ra^WT and Il4ra^ΔIEC mice. Single-cell suspensions were made, and flow cytometry was performed. Cells were gated for viable CD45⁺CD3⁺B220⁻CD4⁺CD62L⁻CD44^high cells, and Gata3⁺ cells were identified as Th2 cells. Percentage of Gata3⁺ cells of the CD62L⁻CD44⁺ population. Scale bar, 500 μm. Data shown are representative of two or more independent experiments, n = 6 biological replicates; statistical tests: t test (C, D, and H–J); two-way ANOVA (E–G); *, P < 0.05; ***, P < 0.005.