Extended Data Fig. 4. Browning of adipose tissues by cold in xenograft and genetically spontaneous tumour models.
a–c, Histological and immunofluorescence staining of BAT in tumour-free mice (a), MMTV-PyMT tumour-bearing mice (b), and ApcMin/+ mice (c) under 30 °C and 4 °C with H&E, UCP1, perilipin, COX4, and CD31, followed by counterstaining with DAPI (blue). Positive signals were randomly quantified (n = 8 or 10 random fields per group). Arrows and arrowheads point to positive signals. d–g, Histological and immunofluorescence staining of sWAT in CRC tumour-bearing mice (d), tumour-free mice (e), MMTV-PyMT tumour-bearing mice (f), and ApcMin/+ mice (g) under 30 °C and 4 °C with H&E, UCP1, perilipin, COX4, and CD31, followed by counterstaining with DAPI (blue). Positive signals were randomly quantified (n = 8 or 10 random fields per group). Arrows and arrowheads point to positive signals. h, Whole-body metabolism of CRC tumour-bearing and tumour-free mice under 30 °C and 4 °C (n = 3 mice per group). All scale bars, 50 μm. Data presented as mean ± s.e.m. Statistical analysis was performed using two-sided unpaired t-test (a–g).