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. 2022 Aug 3;608(7922):421–428. doi: 10.1038/s41586-022-05030-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Blood glucose levels of CRC-tumour-bearing mice after sham and BAT removal under 30 °C or 4 °C. n = 8 mice per group. b, CRC tumour growth rates in sham and BAT-removed mice under 30 °C or 4 °C conditions. n = 10 mice per group. c, Tumour growth curves of BAT-removed MMTV-PyMT mice under 30 °C and 4 °C conditions. n = 6 mice per group. d, Representative PET–CT images and quantification of SUV-BW of BAT-removed MMTV-PyMT mice under 30 °C or 4 °C conditions. n = 6 mice per group. Scale bar, 5 mm. e, Immunofluorescence staining of tumours grown in BAT-removed MMTV-PyMT mice with CA9⁺ hypoxic area, Ki-67⁺ proliferating cells, CD31⁺ microvessels and Cl-Casp3⁺ apoptotic cells. The arrows and arrowheads indicate the respective positive signals. Tumour tissues were counterstained with DAPI (blue). Scale bars, 50 μm. f, Quantification of positive signals of the markers presented in e. n = 6 random fields per group. For a–d and f, data are mean ± s.e.m. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey multiple-comparison test (a and b) and two-sided unpaired t-tests (c, d and f).

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