Fig. 5. High-glucose feeding and UCP1 deficiency abrogate cold-induced tumour suppression.
a, Tumour growth rates of CRC-tumour-bearing mice treated with control vehicle (left) or 15% glucose (right) under 30 °C and 4 °C. n = 5 mice per group. b, Representative PET–CT images and quantification of SUV-BW of tumour-bearing mice with equal tumour size treated with vehicle or 15% glucose under 30 °C and 4 °C. n = 3 mice per group. Scale bars, 5 mm. c, Western blotting detection of non-phosphorylated and phosphorylated PI3K, AKT and GLUT1 of CRC treated with vehicle or 15% glucose under 30 °C or 4 °C. GAPDH was used for standardizing total protein loading levels. d, Tumour growth rates of CRC implanted in WT (left) and Ucp1−/− (right) mice under 30 °C and 4 °C conditions. n = 5 mice per group. e, PET–CT images of 18F-FDG uptake in the BAT and tumours of Ucp1−/− CRC-tumour-bearing mice under 30 °C and 4 °C conditions. Red arrows indicate BAT and orange arrows indicate tumours. Quantification of SUV-BW values of BAT and tumours. n = 3 mice per group. Scale bars, 5 mm. For a, b, d and e, data are mean ± s.e.m. Statistical analysis was performed using one-way ANOVA followed by Tukey multiple-comparison tests (b) or two-sided unpaired t-tests (a, d and e). HG, high glucose (15% glucose).