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. 2022 Aug 3;608(7922):421–428. doi: 10.1038/s41586-022-05030-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Total liver (left) and tumour (right) weights of liver murine CRC tumour-bearing mice under 30 °C and 4 °C (n = 8 mice per group). b, Total liver (left) and tumour (right) weights of liver human CRC tumour-bearing immunodeficient mice under 30 °C and 4 °C (n = 10 mice per group). c-f, Immunofluorescence staining and quantifications of morphology (H&E), CA9⁺ hypoxic area, Ki-67⁺ proliferating cells, CD31⁺ microvessels, Cl-Casp3⁺ apoptotic cells, CD45⁺ inflammatory myeloid cells, IBA1⁺ pan-macrophages, and FSP1⁺ fibroblasts in liver murine CRC (c, d) and liver human CRC (e, f) under 30 °C and 4 °C (n = 5 or 10 random fields per group). Arrows and arrowheads point to positive signals. g, Core and subcutaneous body temperatures of murine CRC subcutaneous tumour-bearing mice under 30 °C and 4 °C (n = 8 mice per group). h, Intra-tumoral temperature of subcutaneous murine CRC tumours under 30 °C and 4 °C (n = 10 tumours per group). i, Core (left) and subcutaneous (right) body temperature of murine CRC tumour-bearing mice in the intra-liver implantation model under 30 °C or 4 °C (n = 8 mice per group). All scale bars, 50 μm. Data presented as mean ± s.e.m. Statistical analysis was performed using two-sided unpaired t-test (a, b, d, f, g–i). NS, not significant.

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