TABLE 2.
Steady-state fluxes in glucose-limited chemostat cultures of E. coli MC4100a
| DOT (%) | Steady-state flux (mmol h−1 g [dry wt]−1)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| qglc | qsucc | qlac | qform | qac | qEtOH | qCO2 | qO2 | |
| 10.0 | −4.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 8.9 | 9.0 |
| 5.0 | −3.8 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 8.6 | 8.4 |
| 2.0 | −3.7 | 0.0 | 0.0 | 0.0 | 0.0 | 0.1 | 8.6 | 8.8 |
| 1.0 | −15.4 | 2.4 | 0.2 | 11.3 | 12.8 | 8.1 | 7.6 | 5.9 |
| 0.5 | −12.5 | 2.3 | 0.2 | 4.8 | 11.3 | 6.9 | 11.2 | 5.0 |
a
Strain MC4100 was grown at D = 0.3 h−1 at pH 6.5. The DOT is given as the percentage of air saturation. The values are the means of at least four independent measurements. The standard deviation is ±10%. Subscripts: glc, glucose; succ, succinate; lac, lactate; form, formate; EtOH, ethanol.