TABLE 3.
In vitro measurements of PDHc in E. coli RM201 and MC4100 cell extracta
Strain | PDHc (nmol of ferricyanide reduced/min/mg of protein)
|
|||
---|---|---|---|---|
Anaerobic | Fumarate | Nitrate | Aerobic | |
RM201 | 26 ± 2.1 | 42 ± 3.1 | 83 ± 4.2 | 131 ± 8.5 |
MC4100 | 22 ± 1.4 | 30 ± 5.3 | 67 ± 4.6 | 128 ± 6.7 |
Cells were obtained from anaerobic glucose-limited chemostat cultures with nitrate (5 mM), with fumarate (25 mM), with no electron acceptor (anaerobic), or aerobically (pH = 6.5, D = 0.1 h−1) as indicated.