TABLE 4.
In vitro measurements of the E2 component of the PDHc in E. coli MC4100, RM201, or RM319 cell extracta
| Strain | E2 component of PDHc (nmol of acetyl lipoate formed/min/mg of protein)
|
|||
|---|---|---|---|---|
| Anaerobic | Fumarate | Nitrate | Aerobic | |
| MC4100 | 24 ± 5.4 | ND | ND | 98 ± 5.8 |
| RM201 | 25 ± 2.1 | 51 ± 4.1 | 85 ± 3.2 | 116 ± 6.2 |
| RM319 | <1 | <1 | <1 | <1 |
a
Cells were obtained from anaerobic chemostat cultures with nitrate (5 mM), with fumarate (12 mM), with no electron acceptor (anaerobic), or in an aerobic chemostat culture as indicated (pH = 6.5, D = 0.1 h−1). ND, not determined.