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. 2022 Aug 10;13:4701. doi: 10.1038/s41467-022-32384-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a PLC reconstituted in large lipid nanodiscs (PLC::MSP2N2) analyzed by size-exclusion chromatography (SEC). The PLC contains two editing modules, centered around the peptide transporter TAP1/2. For better visualization, one of the two editing modules is shown in light grey. b Compositional analysis of human PLC purified by ICP47^SBP in detergent (PLC::GDN) and reconstituted in lipid nanodiscs (PLC::MSP2N2) by SDS-PAGE, immunoblotting, and LC-MS (Supplementary Fig. 1 and 2). c Glycosylation pattern of MHC I allomorph HLA-A*03:01 associated with the PLC (Glc₁Man₉₋₇GlcNAc₂), analyzed by mass spectrometry (deconvoluted spectrum, black). PNGase F-treated sample is shown as reference in cyan. HLA-A*03:01 is the predominant allomorph in LC-MS analysis. d Glycosylation of tapasin in the PLC (Man₉₋₀GlcNAc₂₋₁), analyzed by mass spectrometry (deconvoluted spectrum, black). PNGase F-treated sample is shown as reference in cyan. The data in this figure are representative of two biological replicas. Source data are provided as a Source Data file (a, b) or in the Zenodo open access repository (c, d).