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. 2022 Aug 10;12:13610. doi: 10.1038/s41598-022-17903-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Thickness of photoreceptor inner and outer segments and outer nuclear layer and number of outer nuclear layer cells. (a) Images of representative slices from the control group (n = 4) and H₂ group (n = 4). Photoreceptor inner and outer segments (PR) thickness and outer nuclear layer (ONL) are shown. (b) Photoreceptor inner and outer segments thickness with/without H₂ water. Thickness was significantly greater with H₂ water than without H₂ water (p < 0.01). (c) Outer nuclear layer (ONL) thickness with/without H₂ water. Thickness was significantly greater with H₂ water than without H₂ water (p < 0.01). (d) Outer nuclear layer cells/slide with/without H₂ water. Thickness was significantly greater with H₂ water than without H₂ water (p < 0.05). Bars depict mean ± standard deviation (SD). Scale bar 50 μm. *p < 0.05, **p < 0.01.