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. 2022 Aug 10;12:13610. doi: 10.1038/s41598-022-17903-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of GFAP, MOMA-2, and Iba-1. Immunohistofluorescence for GFAP (a), MOMA-2 (b), and Iba-1 (c) are shown in representative retinal specimens with/without H₂ water (each group: n = 4). We found no significant difference between with/without H₂ water (GFAP: p = 0.64, MOMA-2: p = 0.06). (c) Mean numbers of microglial cells counted in each retinal layer and total. No significant differences between with/without H₂ water were evident. Layers were defined as: ganglion cell layer (GCL); inner plexiform layer (IPL); inner nuclear layer (INL); outer plexiform layer (OPL); outer nuclear layer (ONL); and layer of the photoreceptor outer segments (OS). Scale bar 50 μm. Bars depict means ± standard deviation (SD).