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. 2022 Aug 10;13:4692. doi: 10.1038/s41467-022-32370-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic representation of a chemically induced dimerization (CID) system. FKBP12 and FRB heterodimerize upon the addition of Rapamycin. b Schematic representation of DNAJB1 chemically induced dimers. DNAJB1^ΔDD (teal) and DNAJB1^ΔDD-H244A (dark purple) were expressed as fusion constructs with either FKBP12 or FRB. The addition of Rapamycin facilitates dimer formation of DNAJB1-CID^wt/wt (teal), DNAJB1-CID^H244A/H244A (dark purple), DNAJB1-CID^wt/H244A (mixed protomers) and DNAJB1-CID^H244A/wt (mixed protomers). c Top, FRET analysis of HTTExon1Q₄₈ aggregation upon addition of Hsc70, Apg2, and DNAJB1^wt or DNAJB1-CIDs in the presence of Rapamycin (Rap): DNAJB1-CID^wt/wt (teal), DNAJB1-CID^H244A/H244A (dark purple), DNAJB1-CID^wt/H244A (light blue), DNAJB1-CID^H244A/wt (light purple). Bottom, FRET analysis of HTTExon1Q₄₈ aggregation upon addition of single or double the concentration (2x) of DNAJB1-CIDs in the presence of Rap: DNAJB1-CID^wt/wt (magenta and light purple), DNAJB1-CID^wt/H244A (teal and light blue), DNAJB1-CID^H244A/wt (dark purple and gray).The graphs are a representative result of three independent experiments and a one-way ANOVA analysis of the half-life (T_1/2) of HTTExon1Q₄₈ aggregation under the tested conditions is depicted on the right. Bars represent the mean value and error bars correspond to the mean SD. ****P ≤ 0.0001; ***P ≤ 0.001; **P ≤ 0.01; ns not significant.